Difference between revisions of "Sandbox"

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* '''Description:''' general stress protein, survival of ethanol  stress, spore coat protein <br/><br/>
+
* '''Description:''' deoxyguanosine kinase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yaaH''
+
|''dgk''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yaaG ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein
+
|style="background:#ABCDEF;" align="center"| '''Product''' || deoxyguanosine kinase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || survival of ethanol  stress
+
|style="background:#ABCDEF;" align="center"|'''Function''' || purine salvage and interconversion
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.618  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 5.783  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1281 bp, 427 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 621 bp, 207 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dgk]]'', ''[[yaaI]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dck]]'', ''[[yaaH]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yaaH_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:dgk_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yaaH.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dgk-dck.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10080]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10079]
  
 
=== Additional information===
 
=== Additional information===
Line 72: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' spore coat [http://www.ncbi.nlm.nih.gov/sites/entrez/11964120 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00160]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00150]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
 
required for survival of ethanol stress [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed]
 
  
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''yaaH'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed]
+
* '''Operon:''' ''[[dgk]]-[[dck]]''
 
+
* '''[[Sigma factor]]:'''  
* '''[[Sigma factor]]:''' [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed]
 
  
* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed], expressed during sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed]
+
* '''Regulation:'''  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
Line 120: Line 117:
 
=References=
 
=References=
  
# H&ouml;per et al. (2005) Comprehensive Characterization of the Contribution of Individual [[SigB]]-Dependent General Stress Genes to Stress Resistance of ''Bacillus subtilis''. J. Bact. '''187:''' 2810-2826  [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
# Kodama et al. (1999) The ''Bacillus subtilis yaaH'' gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.''J. Bacteriol.'' '''181:''' 4584-4591. [http://www.ncbi.nlm.nih.gov/sites/entrez/10419957 PubMed]
 
# Höper et al. (2005) Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of ''Bacillus subtilis''.''J.Bacteriol.'' '''187:''' 2810-2826. [http://www.ncbi.nlm.nih.gov/sites/entrez/15805528 PubMed]
 
# Aronson (2002) Sporulation and delta-endotoxin synthesis by ''Bacillus thuringiensis''.''Cell. Mol. Life Sci.'' '''59:''' 417-425. [http://www.ncbi.nlm.nih.gov/sites/entrez/11964120 PubMed]
 

Revision as of 21:43, 15 April 2009

  • Description: deoxyguanosine kinase

Gene name dgk
Synonyms yaaG
Essential no
Product deoxyguanosine kinase
Function purine salvage and interconversion
MW, pI 23 kDa, 5.783
Gene length, protein length 621 bp, 207 aa
Immediate neighbours dck, yaaH
Hier soll was neues rein
Genetic context
Dgk context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed