Difference between revisions of "Sandbox"

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* '''Description:''' RNase M5<br/><br/>
+
* '''Description:''' similar to cell wall binding protein <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''rnmV''
+
|''yabE''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yabF ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || endoribonuclease M5
+
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || 5S ribosomal RNA maturation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 6.681  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 9.282  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 558 bp, 186 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1311 bp, 437 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yabE]]'', ''[[ksgA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yabD]]'', ''[[rnmV]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:rnmV_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yabE_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnmV-ksgA.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yabE.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10104]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10103]
  
 
=== Additional information===
 
=== Additional information===
Line 62: Line 62:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
−
* '''Domains:''' N-terminal part: Toprim domain [http://www.ncbi.nlm.nih.gov/sites/entrez/16077031 PubMed]
+
* '''Domains:''' contains a SPS domain
  
 
* '''Modification:'''
 
* '''Modification:'''
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* '''Interactions:'''
 
* '''Interactions:'''
  
−
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] cytoplasm (homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00410]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00400]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''[[rnmV]]-[[ksgA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]
+
* '''Operon:''' ''yabE'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19047346 PubMed]
  
−
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19047346 PubMed]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''
+
* '''Additional information:''' expression is negatively controlled by a cis-acting antisense RNA that is dependent on [[SigX]] and [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19047346 PubMed]
  
 
=Biological materials =
 
=Biological materials =
Line 113: Line 113:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
−
 
+
[[John Helmann]], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
−
[[Ciaran Condon]], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
 
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
−
# Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in ''Bacillus subtilis'': identification of new proteins at the DNA replication factory ''Proteomics'' '''6:''' 2135-2146. [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
+
# Eiamphungporn W, Helmann JD (2009) Extracytoplasmic function [[sigma factor]]s regulate expression of the ''Bacillus subtilis yabE'' gene via a cis-acting antisense RNA. ''J. Bacteriol.'' '''191:''' 1101-1105. [http://www.ncbi.nlm.nih.gov/sites/entrez/19047346 PubMed]
−
 
 
−
# Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory ''Proteomics'' '''6(7):''' 2135-46. [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
 
−
# Condon et al. (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in ''Bacillus subtilis'': mature 5S rRNA is dispensable for ribosome function. ''RNA'' '''7:''' 242-253. [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]
 
−
# Condon, C., Rourera, J., Brechemier-Baey, D. & Putzer, H. (2002). Ribonuclease M5 has few, if any, mRNA substrates in Bacillus subtilis. J Bacteriol 184, 2845-2849. [http://www.ncbi.nlm.nih.gov/sites/entrez/11976317 PubMed]
 
−
# Allemand et al. (2005) The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member. ''Nucl. Acids Res'' '''33:''' 4368-4376. [http://www.ncbi.nlm.nih.gov/sites/entrez/16077031 PubMed]
 
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 21:41, 15 April 2009

  • Description: similar to cell wall binding protein

Gene name yabE
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 47 kDa, 9.282
Gene length, protein length 1311 bp, 437 aa
Immediate neighbours yabD, rnmV
Hier soll was neues rein
Genetic context
YabE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: contains a SPS domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: expression is negatively controlled by a cis-acting antisense RNA that is dependent on SigX and SigM PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

  1. Eiamphungporn W, Helmann JD (2009) Extracytoplasmic function sigma factors regulate expression of the Bacillus subtilis yabE gene via a cis-acting antisense RNA. J. Bacteriol. 191: 1101-1105. PubMed
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