Difference between revisions of "Sandbox"

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* '''Description:''' transcription repressor of the ''ansAB'' operon<br/><br/>
+
* '''Description:''' L-asparaginase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ansR''
+
|''ansA''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator (Xre family)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || L-asparaginase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || negative regulation of the ''[[ansA]]-[[ansB]]'' operon
+
|style="background:#ABCDEF;" align="center"|'''Function''' || asparagine degradation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 4.807  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 4.455  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 348 bp, 116 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 987 bp, 329 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ansA]]'', ''[[yqxK]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ansB]]'', ''[[ansR]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ansR_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ansA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ansR.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ansAB.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10299]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10300]
  
 
=== Additional information===
 
=== Additional information===
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* '''Protein family:'''
 
* '''Protein family:'''
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[AnsZ]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23590]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23580]
  
* '''E.C. number:'''
+
* '''E.C. number:''' 3.5.1.1
  
 
=== Additional information===
 
=== Additional information===
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''ansR''
+
* '''Operon:''' ''[[ansA]]-[[ansB]]''
  
* '''Sigma factor:'''  
+
* '''Sigma factor:''' [[SigA]]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 118: Line 118:
 
=References=
 
=References=
  
 +
# Sun DX, Setlow P. (1991) Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase. ''J Bacteriol. '' '''Jun;173(12):''' 3831-45. [http://www.ncbi.nlm.nih.gov/sites/entrez/1711029 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 18:54, 15 April 2009

  • Description: L-asparaginase

Gene name ansA
Synonyms
Essential no
Product L-asparaginase
Function asparagine degradation
MW, pI 36 kDa, 4.455
Gene length, protein length 987 bp, 329 aa
Immediate neighbours ansB, ansR
Hier soll was neues rein
Genetic context
AnsA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): AnsZ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number: 3.5.1.1

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Sun DX, Setlow P. (1991) Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase. J Bacteriol. Jun;173(12): 3831-45. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed