Difference between revisions of "Sandbox"

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* '''Description:''' secreted peptide, controls LiaRS activity <br/><br/>
+
* '''Description:''' oxidoreductase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yydF''
+
|''yydG''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || secreted peptide
+
|style="background:#ABCDEF;" align="center"| '''Product''' || oxidoreductase
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of LiaRS activity
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of LiaRS activity
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 5 kDa, 9.567  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.865  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 147 bp, 49 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 957 bp, 319 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yydG]]'', ''[[fbp]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yydH]]'', ''[[yydF]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yydF_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yydG_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yydFGHIJ.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yydFGHIJ.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11479]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11480]
  
 
=== Additional information===
 
=== Additional information===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU40180]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU40170]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 92: Line 92:
 
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
 
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
  
* '''Regulation:''' activated after transition to stationary phase([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
+
* '''Regulation:''' repressed by glucose (5-fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed],  activated after transition to stationary phase([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
  
 
* '''Regulatory mechanism:''' [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
 
* '''Regulatory mechanism:''' [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
  
* '''Additional information:''' the ''yydF'' portion of the mRNA belongs to the most stable mRNAs in ''B. subtilis'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
Line 118: Line 118:
 
=References=
 
=References=
  
 +
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
 
# Butcher BG, Lin YP, Helmann JD (2007) The ''yydFGHIJ'' operon of ''Bacillus subtilis'' encodes a peptide that induces the LiaRS two-component system''J. Bacteriol'' '''189:''' 8616-8625. [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
 
# Butcher BG, Lin YP, Helmann JD (2007) The ''yydFGHIJ'' operon of ''Bacillus subtilis'' encodes a peptide that induces the LiaRS two-component system''J. Bacteriol'' '''189:''' 8616-8625. [http://www.ncbi.nlm.nih.gov/sites/entrez/17921301 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 16:53, 15 April 2009

  • Description: oxidoreductase

Gene name yydG
Synonyms
Essential no
Product oxidoreductase
Function control of LiaRS activity
MW, pI 37 kDa, 5.865
Gene length, protein length 957 bp, 319 aa
Immediate neighbours yydH, yydF
Hier soll was neues rein
Genetic context
YydG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (5-fold) PubMed, activated after transition to stationary phase(AbrB) PubMed
  • Regulatory mechanism: AbrB: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Butcher BG, Lin YP, Helmann JD (2007) The yydFGHIJ operon of Bacillus subtilis encodes a peptide that induces the LiaRS two-component systemJ. Bacteriol 189: 8616-8625. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed