Difference between revisions of "Sandbox"

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* '''Description:''' two-component response regulator, regulation of aerobic and anaerobic respiration <br/><br/>
+
* '''Description:''' RNA polymerase ECF-type [[sigma factor]] SigX <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''resD''
+
|''sigX''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypxD ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypuM ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || two-component response regulator
+
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase ECF-type [[sigma factor]] SigX
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of aerobic and anaerobic respiration <br/>respiration (resA, ctaA, qcrABC, fnr, lctEP)
+
|style="background:#ABCDEF;" align="center"|'''Function''' || cell surface properties
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.631  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 6.086  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 720 bp, 240 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 582 bp, 194 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[resE]]'', ''[[resC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rsiX]]'', ''[[resE]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
Line 24: Line 24:
 
|-
 
|-
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:resD_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:sigX_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
<br/><br/><br/><br/><br/>
+
<br/><br/>
  
 
=The gene=
 
=The gene=
Line 43: Line 43:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/resABCDE.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sigX-rsiX.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10534]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10536]
  
 
=== Additional information===
 
=== Additional information===
Line 59: Line 59:
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
 +
 +
=== Genes controlled by SigX ===
 +
 +
''[[pbpX]]'', ''[[csbB]]'', ''[[abh]]'', ''[[dltA]]-[[dltB]]-[[dltC]]-[[dltD]]-[[dltE]]''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 82: Line 86:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23120]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23100]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 94:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[resA]]-[[resB]]-[[resC]]-[[resD]]-[[resE]]''
+
* '''Operon:''' '' [[sigX]]-[[rsiX]]''
  
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' activated by [[YvrHb]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 120: Line 124:
 
=References=
 
=References=
  
 +
# Huang X, Helmann JD. (1998) Identification of target promoters for the Bacillus subtilis sigma X factor using a consensus-directed search. ''J Mol Biol.'' '''279:''' 165-173. [http://www.ncbi.nlm.nih.gov/sites/entrez/9636707 PubMed]
 +
# Serizawa M, Kodama K, Yamamoto H, Kobayashi K, Ogasawara N, Sekiguchi J. (2005) Functional analysis of the YvrGHb two-component system of ''Bacillus subtilis'': identification of the regulated genes by DNA microarray and northern blot analyses. ''Biosci. Biotechnol. Biochem.'' '''69:''' 2155-2169. [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:16, 15 April 2009

Gene name sigX
Synonyms ypuM
Essential no
Product RNA polymerase ECF-type sigma factor SigX
Function cell surface properties
MW, pI 23 kDa, 6.086
Gene length, protein length 582 bp, 194 aa
Immediate neighbours rsiX, resE
Hier soll was neues rein
Genetic context
SigX context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Genes controlled by SigX

pbpX, csbB, abh, dltA-dltB-dltC-dltD-dltE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Huang X, Helmann JD. (1998) Identification of target promoters for the Bacillus subtilis sigma X factor using a consensus-directed search. J Mol Biol. 279: 165-173. PubMed
  2. Serizawa M, Kodama K, Yamamoto H, Kobayashi K, Ogasawara N, Sekiguchi J. (2005) Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses. Biosci. Biotechnol. Biochem. 69: 2155-2169. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed