Difference between revisions of "Sandbox"

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* '''Description:''' anaerobic coproporphyrinogen III oxidase <br/><br/>
+
* '''Description:''' heat-shock protein (activation of DnaK) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''hemN''
+
|''dnaJ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqeR ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || anaerobic coproporphyrinogen III oxidase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || heat-shock protein (activation of DnaK)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || heme biosynthesis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || protein quality control
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 5.534  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 7.92  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1098 bp, 366 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1116 bp, 372 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hrcA]]'', ''[[lepA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqeT]]'', ''[[surC]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
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|-
 
|-
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:hemN_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:dnaJ_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/lepA-hemN-hrcA-grpE-dnaKJ-yqeTUV.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/lepA-hemN-hrcA-grpE-dnaKJ-yqeTUV.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11395]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10665]
  
 
=== Additional information===
 
=== Additional information===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU25500]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU25460]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[lepA]]-[[hemN]]''
+
* '''Operon:''' ''[[hrcA]]-[[grpE]]-[[dnaK]]-[[dnaJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/1339421 PubMed]
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1339421 PubMed]
  
* '''Regulation:'''  
+
* '''Regulation:''' induced by heat shock [http://www.ncbi.nlm.nih.gov/sites/entrez/1339421 PubMed]
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' repressed at normal temperature by [[HrcA]]
  
* '''Additional information:''' There is a terminator between ''[[lepA]]'' and ''[[hemN]]'' with only little readthrough
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Wolfgang Schumann]], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
 +
# Wetzstein, M., U. Völker, J. Dedio, S. Löbau, U. Zuber, M. Schiesswohl, C. Herget, M. Hecker, and W. Schumann. 1992. Cloning, sequencing, and molecular analysis of the ''dnaK'' locus from ''Bacillus subtilis''. J. Bacteriol. 174:3300-3310. [http://www.ncbi.nlm.nih.gov/sites/entrez/1339421 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:10, 15 April 2009

  • Description: heat-shock protein (activation of DnaK)

Gene name dnaJ
Synonyms
Essential no
Product heat-shock protein (activation of DnaK)
Function protein quality control
MW, pI 40 kDa, 7.92
Gene length, protein length 1116 bp, 372 aa
Immediate neighbours yqeT, surC
Hier soll was neues rein
Genetic context
DnaJ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by heat shock PubMed
  • Regulatory mechanism: repressed at normal temperature by HrcA
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Your additional remarks

References

  1. Wetzstein, M., U. Völker, J. Dedio, S. Löbau, U. Zuber, M. Schiesswohl, C. Herget, M. Hecker, and W. Schumann. 1992. Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J. Bacteriol. 174:3300-3310. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed