Difference between revisions of "Sandbox"

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* '''Description:''' pleiotropic regulator of sulphur metabolism <br/><br/>
+
* '''Description:''' tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''cymR ''
+
|''trmU''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yrzC''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yrrA ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor
+
|style="background:#ABCDEF;" align="center"| '''Product''' || tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sulphur metabolism
+
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 9.879  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 4.729  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 333 bp, 111 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1122 bp, 374 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrvO]]'', ''[[yrvN]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrrB]]'', ''[[yrvO]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yrzC_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:trmU_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 38: Line 38:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 43: Line 45:
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cymR-yrvO-trmU.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cymR-yrvO-trmU.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13813]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13787]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 56:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' Rrf2 family of transcription regulators
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 66: Line 68:
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):''' [[CysK]] acts as corepressor [http://www.ncbi.nlm.nih.gov/sites/entrez/18974048 PubMed]
+
* '''Cofactor(s):'''
 
 
* '''Effectors of protein activity:'''  
 
  
* '''Interactions:''' [[CymR]]-[[CysK]], the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis [http://www.ncbi.nlm.nih.gov/sites/entrez/18974048 PubMed]
+
* '''Effectors of protein activity:'''
  
 +
* '''Interactions:'''
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 81: Line 82:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27520]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27500]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 119: Line 120:
 
=References=
 
=References=
  
# Tanous et al. (2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in ''Bacillus subtilis''.''J. Biol. Chem.'' '''283:''' 35551-35560. [http://www.ncbi.nlm.nih.gov/sites/entrez/18974048 PubMed]
 
# Even S., Burguiere P., Auger S., Soutourina O., Danchin A., Martin-Verstraete I. (2006) Global control of cysteine metabolism by CymR in Bacillus subtilis. ''J. Bacteriol'' '''188:''' 2184-2197  [http://www.ncbi.nlm.nih.gov/sites/entrez/16513748 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:07, 15 April 2009

  • Description: tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase

Gene name trmU
Synonyms yrrA
Essential yes PubMed
Product tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
Function tRNA modification
MW, pI 42 kDa, 4.729
Gene length, protein length 1122 bp, 374 aa
Immediate neighbours yrrB, yrvO
Hier soll was neues rein
Genetic context
TrmU context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed