Difference between revisions of "SinR"

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(Biological materials)
(Biological materials)
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* '''lacZ fusion:'''  
 
* '''lacZ fusion:'''  
 
** pGP1930 (aphA3) based on [[pAC7]], available in [[Jörg Stülke]]'s lab
 
** pGP1930 (aphA3) based on [[pAC7]], available in [[Jörg Stülke]]'s lab
 
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 11:58, 23 July 2015

  • Description: transcriptional regulator (Xre family) of post-exponential-phase responses genes

Gene name sinR
Synonyms sin, flaD
Essential no
Product transcriptional regulator (Xre family)

of post-exponential-phase responses genes

Function control of biofilm formation
Gene expression levels in SubtiExpress: sinR
Interactions involving this protein in SubtInteract: SinR
Metabolic function and regulation of this protein in SubtiPathways:
sinR
MW, pI 12 kDa, 7.177
Gene length, protein length 333 bp, 111 aa
Immediate neighbours sinI, tasA
Sequences Protein DNA DNA_with_flanks
Genetic context
SinR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SinR expression.png















Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

AbrB regulon, ScoC regulon, Spo0A regulon

The SinR regulon

The gene

Basic information

  • Locus tag: BSU24610

Phenotypes of a mutant

  • the mutation suppresses the galactose toxicity to a galE mutant PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Paralogous protein(s): SlrR

Extended information on the protein

  • Kinetic information:
  • Domains:
    • DNA-binding N-terminal domain (aa 1-69) PubMed
    • SinI-binding C-terminal domain (aa 74-111) PubMed
  • Modification:
  • Cofactor(s):

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 3.5 to 13 min) PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 699 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 425 PubMed

Biological materials

  • Expression vector:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Modelling of the SinI/SinR switch

Original publications