NagA

• Description: N-acetylglucosamine-6-phosphate deacetylase
  
  

Gene name	nagA
Synonyms
Essential	no
Product	N-acetylglucosamine-6-phosphate deacetylase
Function	N-acetylglucosamine utilization
Gene expression levels in SubtiExpress: nagA
Metabolic function and regulation of this protein in SubtiPathways: nagA
MW, pI	42 kDa, 5.276
Gene length, protein length	1188 bp, 396 aa
Immediate neighbours	hprK, nagB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, NagR regulon

The gene

Basic information

• Locus tag: BSU35010

Phenotypes of a mutant

• no growth on N-acetylglucosamine PubMed

Database entries

• BsubCyc: BSU35010

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N-acetyl-D-glucosamine 6-phosphate + H₂O = D-glucosamine 6-phosphate + acetate (according to Swiss-Prot)

• Protein family: nagA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(M): 1.4 mM PubMed

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU35010

• Structure: 2VHL PubMed

• UniProt: O34450

• KEGG entry: [2]

• E.C. number: 3.5.1.25

Expression and regulation

• Operon: nagA-nagB-nagR PubMed

• Expression browser: nagA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of N-acetylglucosamine (NagR) PubMed
  • repressed by glucose (2.9-fold) (CcpA) PubMed

• Regulatory mechanism:
  • NagR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 281 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: