Difference between revisions of "DegU"

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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 
** transcription activator and repressor (see [[DegU regulon]])
 
** transcription activator and repressor (see [[DegU regulon]])
** DegU-P represses expression of the fla/che operon in the absence of [[SwrA]], and switches to an activator of the operon in the presence of [[SwrA]] {{PubMed|24386445}}
+
** DegU-P represses expression of the fla/che operon (''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'') in the absence of [[SwrA]], and switches to an activator of the operon in the presence of [[SwrA]] {{PubMed|24386445}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''

Revision as of 14:49, 29 December 2014

Gene name degU
Synonyms sacU, iep
Essential no
Product two-component response regulator
Function regulation of degradative enzymes, genetic competence,

biofilm formation and capsule synthesis

Gene expression levels in SubtiExpress: degU
Interactions involving this protein in SubtInteract: DegU
Metabolic function and regulation of this protein in SubtiPathways:
DegU
MW, pI 25 kDa, 5.602
Gene length, protein length 687 bp, 229 aa
Immediate neighbours yviA, degS
Sequences Protein DNA DNA_with_flanks
Genetic context
DegU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DegU expression.png















Categories containing this gene/protein

biofilm formation, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, DegU regulon, SinR regulon, TnrA regulon

The DegU regulon

The gene

Basic information

  • Locus tag: BSU35490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated by DegS on Asp-56 PubMed, this modulates DNA-binding activity
    • phosphorylation by DegS occurs in response to inhibition of flagellar rotation PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information


Expression and regulation

  • Additional information:
    • DegU-P is degraded by ClpC-ClpP PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1627 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2454 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2251 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1461 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1432 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


The DegU regulon

Original Publications