Difference between revisions of "SinR"
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=References= | =References= | ||
==Reviews== | ==Reviews== | ||
− | <pubmed>20395361 20541494 23353768 </pubmed> | + | <pubmed>20395361 20541494 23353768 24988880 </pubmed> |
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==Modelling of the [[SinI]]/[[SinR]] switch== | ==Modelling of the [[SinI]]/[[SinR]] switch== | ||
<pubmed> 21095906 </pubmed> | <pubmed> 21095906 </pubmed> |
Revision as of 08:15, 7 July 2014
- Description: transcriptional regulator (Xre family) of post-exponential-phase responses genes
Gene name | sinR |
Synonyms | sin, flaD |
Essential | no |
Product | transcriptional regulator (Xre family) of post-exponential-phase responses genes |
Function | control of biofilm formation |
Gene expression levels in SubtiExpress: sinR | |
Interactions involving this protein in SubtInteract: SinR | |
Metabolic function and regulation of this protein in SubtiPathways: sinR | |
MW, pI | 12 kDa, 7.177 |
Gene length, protein length | 333 bp, 111 aa |
Immediate neighbours | sinI, tasA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transcription factors and their control, transition state regulators, biofilm formation
This gene is a member of the following regulons
AbrB regulon, ScoC regulon, Spo0A regulon
The SinR regulon
The gene
Basic information
- Locus tag: BSU24610
Phenotypes of a mutant
Database entries
- BsubCyc: BSU24610
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- transcription regulator of biofilm genes, acts as a true repressor of the tapA-sipW-tasA operon and as an anti-activator (prevents binding of the activator protein RemA) of the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon PubMed
- acts as co-repressor for SlrR PubMed
- Protein family:Xre family
- Paralogous protein(s): SlrR
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU24610
- Structure:
- UniProt: P06533
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 3.5 to 13 min) PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 699 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 425 PubMed
Biological materials
- Mutant:
- GP923 (sinR::spec) PubMed, available in Jörg Stülke's lab
- GP736 (sinR::tetR) PubMed, available in Jörg Stülke's lab
- 1S97 (sinR::phleo), PubMed, available at BGSC
- GP1672 (sinR-tasA::cat) PubMed, available in Jörg Stülke's lab
- GP1663 (yghG-sinI-sinR-tasA), available in Jörg Stülke's lab
- Expression vector:
- N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380: pGP1083 , available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct: GP960 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Modelling of the SinI/SinR switch
Original publications