MelA

• Description: alpha-galactosidase
  
  

Gene name	melA
Synonyms
Essential	no
Product	alpha-galactosidase
Function	melibiose utilization
Gene expression levels in SubtiExpress: melA
Metabolic function and regulation of this protein in SubtiPathways: melA
MW, pI	49 kDa, 5.84
Gene length, protein length	1296 bp, 432 aa
Immediate neighbours	amyC, ytwF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU30300

Database entries

• BsubCyc: BSU30300

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 4 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU30300

• Structure:

• UniProt: O34645

• KEGG entry: [3]

• E.C. number: 3.2.1.22

Expression and regulation

• Operon:
  • msmR-msmE-amyD-amyC-melA PubMed
  • msmE-amyD-amyC-melA PubMed
  • melA PubMed

• Expression browser: melA PubMed

• Sigma factor:
  • msmR: SigA PubMed

• Regulation:
  • MsmR
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 210 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 136 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 817 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: