Difference between revisions of "DapI"
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14190 dapI] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14190 dapI] | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/ | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DapI DapI] |
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=DapI DapI]''' | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 5.102 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 5.102 | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * essential {{PubMed|24261876,12682299}} | |
| − | essential | + | * depletion of DapI results in swelling and rounding up of cells prior to lysis {{PubMed|24261876}} |
=== Database entries === | === Database entries === | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** forms dimers {{PubMed|24261876}} | ||
** [[DapI]]-[[MreB]] {{PubMed|24261876}} | ** [[DapI]]-[[MreB]] {{PubMed|24261876}} | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 274 {{PubMed|24696501}} | ||
| + | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 637 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = | ||
Latest revision as of 11:25, 17 April 2014
- Description: N-acetyl-diaminopimelate deacetylase
| Gene name | ykuR |
| Synonyms | dapL |
| Essential | yes PubMed |
| Product | N-acetyl-diaminopimelate deacetylase |
| Function | biosynthesis of lysine and peptidoglycan |
| Gene expression levels in SubtiExpress: dapI | |
| Interactions involving this protein in SubtInteract: DapI | |
| Metabolic function and regulation of this protein in SubtiPathways: DapI | |
| MW, pI | 41 kDa, 5.102 |
| Gene length, protein length | 1122 bp, 374 aa |
| Immediate neighbours | ykuQ, ykuS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, biosynthesis/ acquisition of amino acids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14190
Phenotypes of a mutant
- essential PubMed
- depletion of DapI results in swelling and rounding up of cells prior to lysis PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: N-acetyl-LL-2,6-diaminopimelate + H2O --> acetate + LL-2,6-diaminopimelate PubMed
- Protein family: peptidase M20A family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- forms uniformly distributed foci in the cytoplasm PubMed
Database entries
- Structure:
- UniProt: O34916
- KEGG entry: [2]
- E.C. number: 3.5.1.47
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Anne-Stéphanie Rueff, Arnaud Chastanet, Julia Domínguez-Escobar, Zhizhong Yao, James Yates, Maria-Victoria Prejean, Olivier Delumeau, Philippe Noirot, Roland Wedlich-Söldner, Sergio R Filipe, Rut Carballido-López
An early cytoplasmic step of peptidoglycan synthesis is associated to MreB in Bacillus subtilis.
Mol Microbiol: 2014, 91(2);348-62
[PubMed:24261876]
[WorldCat.org]
[DOI]
(I p)
