ClpE

• Description: ATP-dependent Clp protease-like (class III stress gene)
  
  

Gene name	clpE
Synonyms	ykvH
Essential	no
Product	ATP-dependent Clp protease-like
Function	protein degradation
Gene expression levels in SubtiExpress: clpE
Interactions involving this protein in SubtInteract: ClpE
Metabolic function and regulation of this protein in SubtiPathways: clpE
MW, pI	77 kDa, 5.135
Gene length, protein length	2097 bp, 699 aa
Immediate neighbours	motA, ykvI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, heat shock proteins

This gene is a member of the following regulons

CtsR regulon

The gene

Basic information

• Locus tag: BSU13700

Database entries

• BsubCyc: BSU13700

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATPase/chaperone

• Protein family: AAA+ -type ATPase (IPR013093) InterPro (PF07724) PFAM

• Paralogous protein(s): ClpC, ClpX

Extended information on the protein

• Kinetic information:

• Domains: AAA-ATPase PFAM

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ClpE-ClpP

• Localization:
  • localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with ClpP Pubmed
  • forms foci coincident with nucleoid edges, usually near cell poles PubMed

Database entries

• BsubCyc: BSU13700

• Structure:

• UniProt: O31673

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: clpE PubMed

• Expression browser: clpE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 165 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 420 PubMed

Biological materials

• Mutant: clpE::spec available from the Hamoen] Lab

• Expression vector:

• lacZ fusion:

• GFP fusion: C-terminal YFP and CFP fusions (single copy) available from the Hamoen] Lab

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Leendert Hamoen, Newcastle University, UK homepage

References

Reviews

Additional reviews: PubMed

Dorte Frees, Kirsi Savijoki, Pekka Varmanen, Hanne Ingmer
Clp ATPases and ClpP proteolytic complexes regulate vital biological processes in low GC, Gram-positive bacteria.
Mol Microbiol: 2007, 63(5);1285-95
[PubMed:17302811] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed