YrhJ

• Description: cytochrome P450 (CYP102A3)/ NADPH-cytochrome P450 reductase
  
  

Gene name	yrhJ
Synonyms	cypE
Essential	no
Product	NADPH-cytochrome P450 reductase
Function	fatty acid metabolism
Gene expression levels in SubtiExpress: yrhJ
Metabolic function and regulation of this protein in SubtiPathways: YrhJ
MW, pI	118 kDa, 6.017
Gene length, protein length	3162 bp, 1054 aa
Immediate neighbours	rrcM, fatR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, lipid metabolism/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

FatR regulon, SigM regulon, SigW regulon, SigX regulon

The gene

Basic information

• Locus tag: BSU27160

Database entries

• BsubCyc: BSU27160

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • hydroxylates medium-chain fatty acids in subterminal positions PubMed

• Protein family:

• Paralogous protein(s): YetO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27160

• Structure: 2X7Y (from Bacillus megaterium; 67% identity, 88% similarity)

• UniProt: O08336

• KEGG entry: [3]

• E.C. number: 1.6.2.4

Expression and regulation

• Operon:
  • fatR-yrhJ PubMed
  • yrhH-fatR-yrhJ PubMed

• Expression browser: yrhJ PubMed

• Sigma factor: SigM PubMed, SigW PubMed, SigX PubMed

• Regulation:
  • induced by unsaturated fatty acids (FatR) PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:
  • FatR: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between yrhK and yrhJ PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: