Difference between revisions of "RnjA"
(→Biological materials) |
|||
Line 59: | Line 59: | ||
=== Database entries === | === Database entries === | ||
+ | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14530&redirect=T BSU14530] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
Line 108: | Line 109: | ||
=== Database entries === | === Database entries === | ||
+ | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14530&redirect=T BSU14530] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3ZQ4 3ZQ4] {{PubMed|21893285}} | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=3ZQ4 3ZQ4] {{PubMed|21893285}} |
Revision as of 13:36, 2 April 2014
- Description: RNase J1
Gene name | rnjA |
Synonyms | ykqC |
Essential | yes PubMed |
Product | RNase J1 |
Function | RNA processing |
Gene expression levels in SubtiExpress: rnjA | |
Interactions involving this protein in SubtInteract: RNase J1 | |
Metabolic function and regulation of this protein in SubtiPathways: rnjA | |
MW, pI | 61 kDa, 5.902 |
Gene length, protein length | 1665 bp, 555 aa |
Immediate neighbours | adeC, ykzG |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14530
Phenotypes of a mutant
- essential PubMed
- a study from the lab of Ciaran Condon reports that rnjA is non-essential and that the mutant is strongly impaired in sporulation, genetic competence and many other phenotypes PubMed
Database entries
- BsubCyc: BSU14530
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: endonuclease and 5'-3' exonuclease
- Protein family: RNase J subfamily (according to Swiss-Prot)
- Paralogous protein(s): RnjB
RNAs affected by rnjA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU14530
- UniProt: Q45493
- KEGG entry: [2]
- E.C. number:
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
- required for thrS RNA processing, involved in maturation of the 5’-end of the16S rRNA
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP41 (rnjA under control of p(xyl)), available in Jörg Stülke's lab
- SSB342 (rnjA under pspac), cat, available in Harald Putzer lab
- Expression vector:
- for chromosomal expression of RNase J1-Strep (spc): GP1034, available in Jörg Stülke's lab
- for chromosomal expression of RNase J1-Strep (cat): GP1042, available in Jörg Stülke's lab
- lacZ fusion: pGP418 (in pAC7), available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1020 (spc, based on pGP1331), available in Jörg Stülke's lab
- GP1075 (aphA3), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Harald Putzer, IBPC Paris, France Homepage
David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage
Your additional remarks
References
Reviews
Original publications