Difference between revisions of "SkfB"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01920&redirect=T BSU01920]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/skf.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/skf.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01920&redirect=T BSU01920]
  
 
* '''Structure:'''
 
* '''Structure:'''

Revision as of 12:52, 2 April 2014

  • Description: thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of spore killing factor

Gene name skfB
Synonyms ybcP, ybcQ
Essential no
Product thioether bond forming radical SAM enzyme
Function maturation of spore killing factor
Gene expression levels in SubtiExpress: skfB
Interactions involving this protein in SubtInteract: SkfB
MW, pI 45 kDa, 4.934
Gene length, protein length 1230 bp, 410 aa
Immediate neighbours skfA, skfC
Sequences Protein DNA DNA_with_flanks
Genetic context
YbcP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SkfB expression.png















Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, prophage 1

This gene is a member of the following regulons

AbrB regulon, PhoP regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU01920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • generates a thioether bond in SkfA, which links the cysteine residue Cys4 with the α-carbon of the methionine residue Met12 PubMed
  • Protein family: moaA/nifB/pqqE family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): contains two [4Fe-4S] clusters PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
    • induced by phosphate starvation (PhoP) PubMed
  • Additional information: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript. The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications

Additional publications: PubMed