NarG

• Description: nitrate reductase (alpha subunit)
  
  

Gene name	narG
Synonyms
Essential	no
Product	nitrate reductase (alpha subunit)
Function	nitrate respiration, nitrogen assimilation
Gene expression levels in SubtiExpress: narG
Interactions involving this protein in SubtInteract: NarG
MW, pI	138 kDa, 6.061
Gene length, protein length	3684 bp, 1228 aa
Immediate neighbours	narH, arfM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration, membrane proteins

This gene is a member of the following regulons

Fnr regulon

The gene

Basic information

• Locus tag: BSU37280

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)

• Protein family: prokaryotic molybdopterin-containing oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • Fe-S cluster PubMed
  • MoCo PubMed

• Effectors of protein activity:

• Interactions:
  • part of a respiratory supercomplex PubMed
  • NarG-NarH PubMed

• Localization:
  • cell membrane PubMed

Database entries

• Structure:

• UniProt: P42175

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: narG-narH-narJ-narI PubMed

• Expression browser: narG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (Fnr) PubMed

• Regulatory mechanism:
  • Fnr: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

D J Richardson, B C Berks, D A Russell, S Spiro, C J Taylor
Functional, biochemical and genetic diversity of prokaryotic nitrate reductases.
Cell Mol Life Sci: 2001, 58(2);165-78
[PubMed:11289299] [WorldCat.org] [DOI] (P p)