Difference between revisions of "NasD"

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(Reviews)
(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[nasB]]-[[nasC]]-[[nasD]]-[[nasE]]-[[nasF]]'' {{PubMed|7836289}}  
+
* '''Operon:'''  
 +
** ''[[nasB]]-[[nasC]]-[[nasD]]-[[nasE]]-[[nasF]]'' {{PubMed|7836289}}
 +
** ''[[nasD]]-[[nasE]]-[[nasF]]'' {{PubMed|9765565}}  
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nasD_355764_358181_-1 nasD] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nasD_355764_358181_-1 nasD] {{PubMed|22383849}}
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* '''Regulation:'''  
 
* '''Regulation:'''  
 
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114,9765565 PubMed]
 
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8799114,9765565 PubMed]
** expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10972836 PubMed]
+
** ''[[nasD]]'': expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10972836 PubMed]
 
** induced by nitric oxide under anaerobic conditions ([[NsrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885456 PubMed]
 
** induced by nitric oxide under anaerobic conditions ([[NsrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885456 PubMed]
  

Revision as of 15:24, 19 March 2014

  • Description: assimilatory nitrite reductase (subunit)

Gene name nasD
Synonyms nasBC, nirB
Essential no
Product assimilatory nitrite reductase (subunit)
Function utilization of nitrite as nitrogen source
Gene expression levels in SubtiExpress: nasD
Interactions involving this protein in SubtInteract: NasD
Metabolic function and regulation of this protein in SubtiPathways:
nasD
MW, pI 88 kDa, 4.948
Gene length, protein length 2415 bp, 805 aa
Immediate neighbours nasE, nasC
Sequences Protein DNA DNA_with_flanks
Genetic context
NasD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NasD expression.png
























Categories containing this gene/protein

respiration, utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

NsrR regulon, ResD regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU03300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H (according to Swiss-Prot)
  • Protein family: nitrite and sulfite reductase 4Fe-4S domain family (according to Swiss-Prot)
  • Paralogous protein(s): NasB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • nasD: expressed under anaerobic conditions (ResD) PubMed
    • induced by nitric oxide under anaerobic conditions (NsrR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original publications