Difference between revisions of "RpoB"
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=== Additional information=== | === Additional information=== | ||
* A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}} | * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}} | ||
| − | + | * mutations in ''[[mtrB]]'', ''[[sigB]]'', ''[[rpoB]]'', and ''[[rpoC]]'' allow ''B. subtilis'' to grow with 4-fluorotryptophan rather than with tryptophan as a canonical amino acid of the genetic code {{PubMed|24572018}} | |
=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
** phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 {{PubMed|22517742}} | ** phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 {{PubMed|22517742}} | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpoB_121919_125500_1 rpoB] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rpoB_121919_125500_1 rpoB] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|7657605}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7657605}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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<pubmed> 22210308 </pubmed> | <pubmed> 22210308 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | + | <pubmed> 19680289 17644585 7657605,12486038,10675340,7592585, 20525796, 17981983, 18763711, 19191541 21512256 21821766 22517742 24572018 22484477,20702425,20817769</pubmed> | |
| − | <pubmed> 19680289 17644585 7657605,12486038,10675340,7592585, 20525796, 17981983, 18763711, 19191541 21512256 21821766 22517742</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:22, 28 February 2014
- Description: RNA polymerase beta subunit
| Gene name | rpoB |
| Synonyms | |
| Essential | yes PubMed |
| Product | RNA polymerase beta subunit |
| Function | transcription |
| Gene expression levels in SubtiExpress: rpoB | |
| Interactions involving this protein in SubtInteract: RpoB | |
| MW, pI | 133 kDa, 4.731 |
| Gene length, protein length | 3579 bp, 1193 aa |
| Immediate neighbours | ybxB, rpoC |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transcription, essential genes, membrane proteins, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01070
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
- mutations in mtrB, sigB, rpoB, and rpoC allow B. subtilis to grow with 4-fluorotryptophan rather than with tryptophan as a canonical amino acid of the genetic code PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)
- Protein family: RNA polymerase beta chain family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 PubMed
- Effectors of protein activity:
- Interactions:
- RpoA-RpoB-RpoC PubMed, NusA-RpoB PubMed
- SigA-(RpoB-RpoC) PubMed, SigB-(RpoB-RpoC)
- SigD-(RpoB-RpoC), SigE-(RpoB-RpoC)
- SigF-(RpoB-RpoC), SigG-(RpoB-RpoC)
- SigH-(RpoB-RpoC), SigI-(RpoB-RpoC)
- SigK-(RpoB-RpoC), SigL-(RpoB-RpoC)
- SigM-(RpoB-RpoC), SigV-(RpoB-RpoC)
- SigW-(RpoB-RpoC), SigX-(RpoB-RpoC)
- SigY-(RpoB-RpoC), SigZ-(RpoB-RpoC)
- Xpf-(RpoB-RpoC), YlaC-(RpoB-RpoC)
- YvrI-RpoB PubMed,
- Mfd-RpoB PubMed
- Localization: membrane associated PubMed
Database entries
- Structure:
- UniProt: P37870
- KEGG entry: [3]
- E.C. number: 2.7.7.6
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Operon: rpoB DBTBS
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
