Difference between revisions of "IlvD"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ilvD_2300762_2302438_-1 ilvD] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ilvD_2300762_2302438_-1 ilvD] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | * '''lacZ fusion:''' pGP522 (in [[pAC5]]), pGP235 (in [[pAC5]]), both available in [[Stülke]] lab; a series of promoter deletions in [[pAC6]] is available in [[Stülke]] lab | + | * '''lacZ fusion:''' pGP522 (in [[pAC5]]), pGP235 (in [[pAC5]]), both available in [[Jörg Stülke]]'s lab; a series of promoter deletions in [[pAC6]] is available in [[Jörg Stülke]]'s lab |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
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=References= | =References= | ||
| − | <pubmed>18083814,12618455,15060025,12107147, </pubmed> | + | <pubmed>18083814,12618455,15060025,12107147, 24163341</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:22, 22 December 2013
- Description: dihydroxy-acid dehydratase (2,3-dihydroxy-3-methylbutanoate, 2,3-dihydroxy-3-methylpentanoate)
| Gene name | ilvD |
| Synonyms | |
| Essential | no |
| Product | dihydroxy-acid dehydratase (2,3-dihydroxy-3-methylbutanoate, 2,3-dihydroxy-3-methylpentanoate) |
| Function | biosynthesis of branched-chain amino acids |
| Gene expression levels in SubtiExpress: ilvD | |
| Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A | |
| MW, pI | 59 kDa, 5.315 |
| Gene length, protein length | 1674 bp, 558 aa |
| Immediate neighbours | brxA, ypgR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
| Expression at a glance PubMed 500px | |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU21870
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P51785
- KEGG entry: [3]
- E.C. number: 4.2.1.9
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion: pGP522 (in pAC5), pGP235 (in pAC5), both available in Jörg Stülke's lab; a series of promoter deletions in pAC6 is available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
