CtpB

• Description: carboxy-terminal processing serine protease, cleaves SpoIVFA, this results in processing of pro-SigK
  
  

Gene name	ctpB
Synonyms	yvjB
Essential	no
Product	carboxy-terminal processing serine protease, cleaves SpoIVFA
Function	control of SigK activation
Gene expression levels in SubtiExpress: ctpB
Interactions involving this protein in SubtInteract: CtpB
MW, pI	52 kDa, 8.641
Gene length, protein length	1440 bp, 480 aa
Immediate neighbours	swrAA/2, ftsX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU35240

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): CtpA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • substrate binding of CtpB induces opening of the PDZ gate and protease activation PubMed
  • CtpB acts in concert with a second signaling protease, SpoIVB PubMed

• Interactions:
  • CtpB-SpoIVFA PubMed

• Localization:
  • intracellular space between the mother cell and the forespore PubMed

Database entries

• Structure:
  • forms a ring-like scaffold with two narrow tunnels for substrate binding PubMed

• UniProt: O35002

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctpB (according to DBTBS)

• Expression browser: ctpB PubMed

• Sigma factor: SigE PubMed, SigG PubMed

• Regulation: expressed during sporulation (SigE, SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S129 ( ctpB::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Markus Mastny, Alexander Heuck, Robert Kurzbauer, Anja Heiduk, Prisca Boisguerin, Rudolf Volkmer, Michael Ehrmann, Christopher D A Rodrigues, David Z Rudner, Tim Clausen
CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis.
Cell: 2013, 155(3);647-58
[PubMed:24243021] [WorldCat.org] [DOI] (I p)

Nathalie Campo, David Z Rudner
SpoIVB and CtpB are both forespore signals in the activation of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2007, 189(16);6021-7
[PubMed:17557826] [WorldCat.org] [DOI] (P p)

Nathalie Campo, David Z Rudner
A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.
Mol Cell: 2006, 23(1);25-35
[PubMed:16818230] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Qi Pan, Richard Losick, David Z Rudner
A second PDZ-containing serine protease contributes to activation of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2003, 185(20);6051-6
[PubMed:14526016] [WorldCat.org] [DOI] (P p)