Difference between revisions of "OdhB"
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* '''Mutant:''' | * '''Mutant:''' | ||
+ | ** GP1276 (''[[odhA]]-[[odhB]]''::''cat''), available in [[Jörg Stülke]]'s lab | ||
* '''Expression vector:''' | * '''Expression vector:''' |
Revision as of 14:10, 13 August 2013
- Description: 2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)
Gene name | odhB |
Synonyms | citM |
Essential | yes PubMed |
Product | 2-oxoglutarate dehydrogenase complex
(dihydrolipoamide transsuccinylase, E2 subunit) |
Function | TCA cycle |
Gene expression levels in SubtiExpress: odhB | |
Interactions involving this protein in SubtInteract: OdhB | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 45 kDa, 4.859 |
Gene length, protein length | 1251 bp, 417 aa |
Immediate neighbours | yocS, odhA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, essential genes, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU19360
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine (according to Swiss-Prot)
- Protein family: sodium:bile acid symporter family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- lipoic acid
- Effectors of protein activity:
- Localization:
- membrane associated PubMed
Database entries
- UniProt: P16263
- KEGG entry: [3]
- E.C. number: 2.3.1.61
Additional information
- extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- GP1276 (odhA-odhB::cat), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications