PucL

• Description: uricase, urate oxidase
  
  

Gene name	pucL
Synonyms	yunL
Essential	no
Product	uricase
Function	purine utilization
Gene expression levels in SubtiExpress: pucL
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	56 kDa, 5.484
Gene length, protein length	1482 bp, 494 aa
Immediate neighbours	pucK, pucM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU32450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO₂ (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1J2G (with 8-Azaxanthine from Bacillus sp., 66% identity, 79% similarity)

• UniProt: O32141

• KEGG entry: [3]

• E.C. number: 1.7.3.3

Expression and regulation

• Operon:
  • pucR-pucJ-pucK-pucL-pucM PubMed
  • pucJ-pucK-pucL-pucM PubMed

• Expression browser: pucL PubMed

• Sigma factor:
  • pucJ: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: