Difference between revisions of "Sat"

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Revision as of 13:18, 16 May 2013

  • Description: sulfate adenylyltransferase

Gene name sat
Synonyms ylnB
Essential no
Product sulfate adenylyltransferase
Function sulfate activation
Gene expression levels in SubtiExpress: sat
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 42 kDa, 5.646
Gene length, protein length 1146 bp, 382 aa
Immediate neighbours cysP, cysC
Sequences Protein DNA DNA_with_flanks
Genetic context
Sat context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Sat expression.png




























Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CymR regulon, S-box

The gene

Basic information

  • Locus tag: BSU15590

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + sulfate = diphosphate + adenylyl sulfate (according to Swiss-Prot)
  • Protein family: sulfate adenylyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s): YitA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR)
    • induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism:
    • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
    • CymR: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References