MccA

• Description: O-acetylserine-thiol-lyase
  
  

Gene name	mccA
Synonyms	yrhA
Essential	no
Product	O-acetylserine-thiol-lyase
Function	methionine-to-cysteine conversion
Gene expression levels in SubtiExpress: mccA
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	32 kDa, 5.227
Gene length, protein length	921 bp, 307 aa
Immediate neighbours	mccB, mtnN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CymR regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU27260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: O(3)-acetyl-L-serine + L-homocysteine = L-cystathionine + acetate (according to Swiss-Prot)

• Protein family: cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O05393

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yrrT-mtnN-mccA-mccB-yrhC PubMed

• Expression browser: mccA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A945 ( mccA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: