Difference between revisions of "YkuI"
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuH]]'', ''[[fsrA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuH]]'', ''[[fsrA]]'' | ||
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| − | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14090 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14090 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14090 | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14090 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14090 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14090 DNA_with_flanks] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ykuI_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ykuI_context.gif]] | ||
Revision as of 10:04, 14 May 2013
- Description: potential c-di-GMP degrading phosphodiesterase
| Gene name | ykuI |
| Synonyms | |
| Essential | no |
| Product | potential c-di-GMP degrading phosphodiesterase |
| Function | unknown |
| Gene expression levels in SubtiExpress: ykuI | |
| MW, pI | 47 kDa, 4.462 |
| Gene length, protein length | 1221 bp, 407 aa |
| Immediate neighbours | ykuH, fsrA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14090
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: degradation of cyclic di-GMP PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: EAL domain (aa 1 ... 245, for c-di-GMP hydrolysis), PAS-like domain (aa 300 ... 400) PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O35014
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1324 (tet), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
George Minasov, Sivaraman Padavattan, Ludmilla Shuvalova, Joseph S Brunzelle, Darcie J Miller, Arnaud Baslé, Claudia Massa, Frank R Collart, Tilman Schirmer, Wayne F Anderson
Crystal structures of YkuI and its complex with second messenger cyclic Di-GMP suggest catalytic mechanism of phosphodiester bond cleavage by EAL domains.
J Biol Chem: 2009, 284(19);13174-84
[PubMed:19244251]
[WorldCat.org]
[DOI]
(P p)
