Difference between revisions of "RocF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || arginine utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || arginine utilization
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU40320 rocF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40320 rocF]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[phrG]]'', ''[[rocE]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[phrG]]'', ''[[rocE]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB16069&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40320 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:rocF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:rocF_context.gif]]

Revision as of 15:19, 13 May 2013

  • Description: arginase

Gene name rocF
Synonyms
Essential no
Product arginase
Function arginine utilization
Gene expression levels in SubtiExpress: rocF
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 32 kDa, 4.932
Gene length, protein length 888 bp, 296 aa
Immediate neighbours phrG, rocE
Sequences Protein DNA Advanced_DNA
Genetic context
RocF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RocF expression.png















Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU40320

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-arginine + H2O = L-ornithine + urea (according to Swiss-Prot)
  • Protein family: arginase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Ser-68 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2EF5 (from Thermus thermophilus, 46% identity, 63% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by arginine (RocR, AhrC) PubMed
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant: GP655, aphA3, available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References