Difference between revisions of "PucL"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization | |style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32450 pucL] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucK]]'', ''[[pucM]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucK]]'', ''[[pucM]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32450 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:pucL_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:pucL_context.gif]] | ||
Revision as of 13:46, 13 May 2013
- Description: uricase
| Gene name | pucL |
| Synonyms | yunL |
| Essential | no |
| Product | uricase |
| Function | purine utilization |
| Gene expression levels in SubtiExpress: pucL | |
| Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation) | |
| MW, pI | 56 kDa, 5.484 |
| Gene length, protein length | 1482 bp, 494 aa |
| Immediate neighbours | pucK, pucM |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU32450
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO2 (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1J2G (with 8-Azaxanthine from Bacillus sp., 66% identity, 79% similarity)
- UniProt: O32141
- KEGG entry: [3]
- E.C. number: 1.7.3.3
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Pollyana Pfrimer, Lidia Maria Pepe de Moraes, Alexsandro Sobreira Galdino, Loise Pedrosa Salles, Viviane Castelo Branco Reis, Janice Lisboa De Marco, Maura Vianna Prates, Carlos Bloch, Fernando Araripe Gonçalves Torres
Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli.
J Biomed Biotechnol: 2010, 2010;674908
[PubMed:20168977]
[WorldCat.org]
[DOI]
(I p)
Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818]
[WorldCat.org]
[DOI]
(P p)
Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039]
[WorldCat.org]
[DOI]
(P p)
A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136]
[WorldCat.org]
[DOI]
(P p)
