Difference between revisions of "YlnE"

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|style="background:#ABCDEF;" align="center"|'''Function''' || siroheme biosynthesis, sulfite reduction
 
|style="background:#ABCDEF;" align="center"|'''Function''' || siroheme biosynthesis, sulfite reduction
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU15620 ylnE]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15620 ylnE]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylnD]]'', ''[[ylnF]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylnD]]'', ''[[ylnF]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13436&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15620 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15620 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15620 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ylnE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ylnE_context.gif]]

Revision as of 12:47, 13 May 2013

  • Description: probable siroheme ferrochelatase

Gene name ylnE
Synonyms
Essential no
Product probable siroheme ferrochelatase
Function siroheme biosynthesis, sulfite reduction
Gene expression levels in SubtiExpress: ylnE
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 28 kDa, 6.063
Gene length, protein length 783 bp, 261 aa
Immediate neighbours ylnD, ylnF
Sequences Protein DNA Advanced_DNA
Genetic context
YlnE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YlnE expression.png




























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

CymR regulon, S-box

The gene

Basic information

  • Locus tag: BSU15620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Siroheme + 2 H+ = sirohydrochlorin + Fe2+ (according to Swiss-Prot)
  • Protein family: SirB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR)
    • induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism:
    • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
    • CymR: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References