Difference between revisions of "CitM"
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|style="background:#ABCDEF;" align="center"|'''Function''' || citrate uptake | |style="background:#ABCDEF;" align="center"|'''Function''' || citrate uptake | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07610 citM] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://cellpublisher.gobics.de/view/GqbblRJkBH Metal ion homeostasis]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflP]]'', ''[[yflN]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflP]]'', ''[[yflN]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU07610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU07610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU07610 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:citM_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:citM_context.gif]] | ||
Revision as of 12:21, 13 May 2013
- Description: Mg2+-citrate transporter
| Gene name | citM |
| Synonyms | yflO |
| Essential | no |
| Product | Mg2+-citrate transporter |
| Function | citrate uptake |
| Gene expression levels in SubtiExpress: citM | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Metal ion homeostasis | |
| MW, pI | 45 kDa, 6.227 |
| Gene length, protein length | 1299 bp, 433 aa |
| Immediate neighbours | yflP, yflN |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
transporters/ other, utilization of specific carbon sources, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU07610
Phenotypes of a mutant
no growth with citrate as sole carbon source PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: View classification (according to Swiss-Prot)
- Paralogous protein(s): CitH
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P55069
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538]
[WorldCat.org]
[DOI]
(I e)
Guillermo D Repizo, Víctor S Blancato, Pablo D Sender, Juke Lolkema, Christian Magni
Catabolite repression of the citST two-component system in Bacillus subtilis.
FEMS Microbiol Lett: 2006, 260(2);224-31
[PubMed:16842348]
[WorldCat.org]
[DOI]
(P p)
Jessica B Warner, Juke S Lolkema
A Crh-specific function in carbon catabolite repression in Bacillus subtilis.
FEMS Microbiol Lett: 2003, 220(2);277-80
[PubMed:12670692]
[WorldCat.org]
[DOI]
(P p)
Jessica B Warner, Christian Magni, Juke S Lolkema
CcpA-independent regulation of expression of the Mg2+ -citrate transporter gene citM by arginine metabolism in Bacillus subtilis.
J Bacteriol: 2003, 185(3);854-9
[PubMed:12533460]
[WorldCat.org]
[DOI]
(P p)
Jessica B Warner, Juke S Lolkema
Growth of Bacillus subtilis on citrate and isocitrate is supported by the Mg2+-citrate transporter CitM.
Microbiology (Reading): 2002, 148(Pt 11);3405-3412
[PubMed:12427932]
[WorldCat.org]
[DOI]
(P p)
Bastiaan P Krom, Henry Huttinga, Jessica B Warner, Juke S Lolkema
Impact of the Mg(2+)-citrate transporter CitM on heavy metal toxicity in Bacillus subtilis.
Arch Microbiol: 2002, 178(5);370-5
[PubMed:12375105]
[WorldCat.org]
[DOI]
(P p)
H Li, A M Pajor
Functional characterization of CitM, the Mg2+-citrate transporter.
J Membr Biol: 2002, 185(1);9-16
[PubMed:11891560]
[WorldCat.org]
[DOI]
(P p)
B P Krom, J B Warner, W N Konings, J S Lolkema
Complementary metal ion specificity of the metal-citrate transporters CitM and CitH of Bacillus subtilis.
J Bacteriol: 2000, 182(22);6374-81
[PubMed:11053381]
[WorldCat.org]
[DOI]
(P p)
J B Warner, B P Krom, C Magni, W N Konings, J S Lolkema
Catabolite repression and induction of the Mg(2+)-citrate transporter CitM of Bacillus subtilis.
J Bacteriol: 2000, 182(21);6099-105
[PubMed:11029430]
[WorldCat.org]
[DOI]
(P p)
H Yamamoto, M Murata, J Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Mol Microbiol: 2000, 37(4);898-912
[PubMed:10972810]
[WorldCat.org]
[DOI]
(P p)
A Boorsma, M E van der Rest, J S Lolkema, W N Konings
Secondary transporters for citrate and the Mg(2+)-citrate complex in Bacillus subtilis are homologous proteins.
J Bacteriol: 1996, 178(21);6216-22
[PubMed:8892821]
[WorldCat.org]
[DOI]
(P p)
P Carlsson, L Hederstedt
Bacillus subtilis citM, the structural gene for dihydrolipoamide transsuccinylase: cloning and expression in Escherichia coli.
Gene: 1987, 61(2);217-24
[PubMed:3127276]
[WorldCat.org]
[DOI]
(P p)
