Difference between revisions of "GuaA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of GMP | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of GMP | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06360 guaA] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purine_biosynthesis.html Purine synthesis], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purine_biosynthesis.html Purine synthesis], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yebA]]'', ''[[pbuG]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yebA]]'', ''[[pbuG]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06360 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06360 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06360 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:guaA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:guaA_context.gif]] | ||
Revision as of 12:17, 13 May 2013
- Description: GMP synthase (glutamine-hydrolysing)
| Gene name | guaA |
| Synonyms | guaB |
| Essential | no |
| Product | GMP synthetase (glutamine-hydrolysing) |
| Function | biosynthesis of GMP |
| Gene expression levels in SubtiExpress: guaA | |
| Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation) | |
| MW, pI | 57 kDa, 4.747 |
| Gene length, protein length | 1539 bp, 513 aa |
| Immediate neighbours | yebA, pbuG |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06360
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- the enzyme is inhibited by guanosine tetraphosphate (stringent response) PubMed
Database entries
- Structure: 2YWB (from Thermus thermophilus hb8, 45% identity, 57% similarity)
- UniProt: P29727
- KEGG entry: [3]
- E.C. number: 6.3.5.2
Additional information
Expression and regulation
- Operon: guaA PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
P Mäntsälä, H Zalkin
Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP.
J Bacteriol: 1992, 174(6);1883-90
[PubMed:1312531]
[WorldCat.org]
[DOI]
(P p)
H H Saxild, P Nygaard
Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools.
J Gen Microbiol: 1991, 137(10);2387-94
[PubMed:1722815]
[WorldCat.org]
[DOI]
(P p)
T Mitani, J E Heinze, E Freese
Induction of sporulation in Bacillus subtilis by decoyinine or hadacidin.
Biochem Biophys Res Commun: 1977, 77(3);1118-25
[PubMed:409404]
[WorldCat.org]
[DOI]
(P p)
