Difference between revisions of "CdaA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of c-di-AMP | |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of c-di-AMP | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01750 cdaA] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CdaA CdaA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CdaA CdaA] | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rsiW]]'', ''[[cdaR]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rsiW]]'', ''[[cdaR]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01750 Advanced_DNA] |
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Revision as of 12:00, 13 May 2013
- Description: diadenylate cyclase, synthesis of c-di-AMP in vegetative cells
| Gene name | cdaA |
| Synonyms | ybbP, ybbQ |
| Essential | no |
| Product | diadenylate cyclase |
| Function | synthesis of c-di-AMP |
| Gene expression levels in SubtiExpress: cdaA | |
| Interactions involving this protein in SubtInteract: CdaA | |
| Metabolic function and regulation of this protein in SubtiPathways: Murein recycling | |
| MW, pI | 30 kDa, 8.074 |
| Gene length, protein length | 819 bp, 273 aa |
| Immediate neighbours | rsiW, cdaR |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
membrane proteins, metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01750
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-AMP from two molecules of ATP PubMed
- Protein family:
- Paralogous protein(s): CdaS
Extended information on the protein
- Kinetic information:
- Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: Q45589
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- GP997 (cdaA::cat), available in Jörg Stülke's lab
- GP985 (cdaA-cdaR::cat), available in Jörg Stülke's lab
- Expression vector:
- expression of native cdaA in B. subtilis: pGP1960 (in pBQ200), available in Jörg Stülke's lab
- expression of cdaA-Strep in B. subtilis suitable for SPINE: pGP1986 (in pGP382), available in Jörg Stülke's lab
- lacZ fusion: GP1339 (cat) based on pAC6, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmid: pGP1990.
- FLAG-tag construct:
- GP1381 cdaA-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Additional publications: PubMed
Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352]
[WorldCat.org]
[DOI]
(I p)
Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650]
[WorldCat.org]
[DOI]
(I p)
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
