Difference between revisions of "YvcK"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * unable to grow with gluconeogenic substrates as single carbon source {{PubMed|16272399}} | |
| − | + | * filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) {{PubMed|16272399}}, this is supressed by overexpression of [[MreB]] or by deletion of ''[[ponA]]'' ([[ponA|PBP1]] ) {{PubMed|21320184}} | |
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
Revision as of 19:47, 29 April 2013
- Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
| Gene name | yvcK |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | localization of PBP1 |
| Gene expression levels in SubtiExpress: yvcK | |
| MW, pI | 34 kDa, 5.408 |
| Gene length, protein length | 951 bp, 317 aa |
| Immediate neighbours | yvcL, yvcJ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
carbon core metabolism, cell shape
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34760
Phenotypes of a mutant
- unable to grow with gluconeogenic substrates as single carbon source PubMed
- filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UPF0052 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: localized as a helical-like pattern PubMed
Database entries
- Structure:
- UniProt: O06974
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
- translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
- Mutant:
- Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Boris Görke, University of Göttingen, Germany Homepage
Anne Galinier, University of Marseille, France
Your additional remarks
References
Reviews
Original publications
Elodie Foulquier, Frédérique Pompeo, Alain Bernadac, Leon Espinosa, Anne Galinier
The YvcK protein is required for morphogenesis via localization of PBP1 under gluconeogenic growth conditions in Bacillus subtilis.
Mol Microbiol: 2011, 80(2);309-18
[PubMed:21320184]
[WorldCat.org]
[DOI]
(I p)
Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading): 2005, 151(Pt 11);3777-3791
[PubMed:16272399]
[WorldCat.org]
[DOI]
(P p)
A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc Natl Acad Sci U S A: 1997, 94(16);8439-44
[PubMed:9237995]
[WorldCat.org]
[DOI]
(P p)
