Difference between revisions of "RocG"
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==Expression of ''rocG''== | ==Expression of ''rocG''== | ||
− | '''Additional publications:''' {{PubMed|20817675}} | + | '''Additional publications:''' {{PubMed|22900538,20817675}} |
− | <pubmed>12634342,15150224 10468601 9829940 | + | <pubmed>12634342,15150224 10468601 9829940 </pubmed> |
==Structural analysis of glutamate dehydrogenase== | ==Structural analysis of glutamate dehydrogenase== |
Revision as of 11:38, 23 January 2013
- Description: trigger enzyme: catabolic glutamate dehydrogenase induced by arginine, ornithine or proline, subject to carbon catabolite repression
Gene name | rocG |
Synonyms | |
Essential | no |
Product | trigger enzyme: glutamate dehydrogenase (major) |
Function | arginine utilization, controls the activity of GltC |
Gene expression levels in SubtiExpress: rocG | |
Interactions involving this protein in SubtInteract: RocG | |
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
MW, pI | 46.2 kDa, 6.28 |
Gene length, protein length | 1272 bp, 424 amino acids |
Immediate neighbours | rocA, yweA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
utilization of amino acids, glutamate metabolism, transcription factors and their control, trigger enzyme
This gene is a member of the following regulons
AbrB regulon, AhrC regulon, CcpA regulon, RocR regulon, SigL regulon
The gene
Basic information
- Locus tag: BSU37790
Phenotypes of a mutant
- Poor growth on complex media such as SP (sporulation medium). No growth in minimal media with arginine as the only carbon source. Rapid accumulation of suppressor mutants (gudB1)
- sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin (suppressed by activation of gudB) due to the downregulation of the SigW regulon PubMed
- transcription profile of a rocG gudB mutant strain: GEO PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH + H+ (according to Swiss-Prot), controls the activity of the GltC transcription activator PubMed
- Protein family: Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)
- Paralogous protein(s): GudB
Extended information on the protein
- Kinetic information: KM [glutamate] = 2.9 mM, KM [ammonium] = 18 mM PubMed
- Domains:
- Modification:
- Cofactor(s): NAD+/NADH + H+
- Effectors of protein activity:
Database entries
- Structure: 3K92 (super-repressor mutant that is capable of constitutive inactivation of GltC, E93K mutation) PubMed
- UniProt: P39633
- KEGG entry: [4]
- E.C. number: 1.4.1.2
Additional information
Expression and regulation
- Operon: rocG PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Activation by RocR requires binding of RocR to a downstream element PubMed
Biological materials
- Mutant: GP747 (spc), GP726 (aphA3), GP810 (del tet), GP1157 (cat) all available in Stülke lab
- Expression vector:
- expression of native rocG in B. subtilis: pGP529 (in pBQ200), available in Stülke lab
- for purification of RocG from E. coli carrying an N-terminal Strep-tag: pGP902 (in pGP172), a series of rocG variants is also available in pGP172, available in Stülke lab
- for expression/ purification from E. coli with N-terminal His-tag and thrombin cleavage site, in pWH844: pGP860, available in Stülke lab
- purification from B. subtilis with an N-terminal Strep-tag, for SPINE, (in pGP380): pGP1709, available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody: available in Stülke lab
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
Fabian Commichau University of Göttingen, Germany Homepage
Your additional remarks
References
Reviews
Gunka K, Commichau FM Control of glutamate homeostasis in Bacillus subtilis: a complex interplay between ammonium assimilation, glutamate biosynthesis and degradation. Mol Microbiol.: 2012, 85(2) 213-224. PubMed:22625175
Jason R Treberg, Margaret E Brosnan, Malcolm Watford, John T Brosnan
On the reversibility of glutamate dehydrogenase and the source of hyperammonemia in the hyperinsulinism/hyperammonemia syndrome.
Adv Enzyme Regul: 2010, 50(1);34-43
[PubMed:19895831]
[WorldCat.org]
[DOI]
(I p)
Victoria I Bunik, Alisdair R Fernie
Metabolic control exerted by the 2-oxoglutarate dehydrogenase reaction: a cross-kingdom comparison of the crossroad between energy production and nitrogen assimilation.
Biochem J: 2009, 422(3);405-21
[PubMed:19698086]
[WorldCat.org]
[DOI]
(I e)
N M Brunhuber, J S Blanchard
The biochemistry and enzymology of amino acid dehydrogenases.
Crit Rev Biochem Mol Biol: 1994, 29(6);415-67
[PubMed:7705101]
[WorldCat.org]
[DOI]
(P p)
R C Hudson, R M Daniel
L-glutamate dehydrogenases: distribution, properties and mechanism.
Comp Biochem Physiol B: 1993, 106(4);767-92
[PubMed:8299344]
[WorldCat.org]
[DOI]
(P p)
Enzymatic activity of RocG
Function in the control of GltC activity
Expression of rocG
Additional publications: PubMed
Structural analysis of glutamate dehydrogenase
Bypass of rocG mutations
Katrin Gunka, Stefan Tholen, Jan Gerwig, Christina Herzberg, Jörg Stülke, Fabian M Commichau
A high-frequency mutation in Bacillus subtilis: requirements for the decryptification of the gudB glutamate dehydrogenase gene.
J Bacteriol: 2012, 194(5);1036-44
[PubMed:22178973]
[WorldCat.org]
[DOI]
(I p)
Lope A Flórez, Katrin Gunka, Rafael Polanía, Stefan Tholen, Jörg Stülke
SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants.
BMC Syst Biol: 2011, 5;5
[PubMed:21219666]
[WorldCat.org]
[DOI]
(I e)