Difference between revisions of "CdaA"
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* '''FLAG-tag construct:''' | * '''FLAG-tag construct:''' | ||
− | ** GP1331 | + | ** GP1331 ''lacA::cdaR-Strep aphA3 cdaA-3xFLAG ermC'' (based on [[pGP1460]] and [[pGP1087]]), available in [[Jörg Stülke]]'s lab |
− | ** GP1381 cdaA-3xFLAG ermC (based on [[ | + | ** GP1381 ''cdaA-3xFLAG ermC'' (based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' |
Revision as of 13:54, 18 December 2012
- Description: diadenylate cyclase, synthesis of c-di-AMP in vegetative cells
Gene name | cdaA |
Synonyms | ybbP, ybbQ |
Essential | no |
Product | diadenylate cyclase |
Function | synthesis of c-di-AMP |
Gene expression levels in SubtiExpress: cdaA | |
Interactions involving this protein in SubtInteract: CdaA | |
Metabolic function and regulation of this protein in SubtiPathways: Murein recycling | |
MW, pI | 30 kDa, 8.074 |
Gene length, protein length | 819 bp, 273 aa |
Immediate neighbours | rsiW, cdaR |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
membrane proteins, metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01750
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-AMP from two molecules of ATP PubMed
- Protein family:
- Paralogous protein(s): CdaS
Extended information on the protein
- Kinetic information:
- Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: Q45589
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- GP997 (cdaA::cat), available in Jörg Stülke's lab
- GP985 (cdaA-cdaR::cat), available in Jörg Stülke's lab
- Expression vector:
- expression of native cdaA in B. subtilis: pGP1960 (in pBQ200), available in Jörg Stülke's lab
- expression of cdaA-Strep in B. subtilis suitable for SPINE: pGP1986 (in pGP382), available in Jörg Stülke's lab
- lacZ fusion: GP1339 (cat) based on pAC6, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmid: pGP1990.
- FLAG-tag construct:
- GP1331 lacA::cdaR-Strep aphA3 cdaA-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab
- GP1381 cdaA-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Additional publications: PubMed