YngJ

• Description: 3-methylbutanoyl-CoA dehydrogenase
  
  

Gene name	yngJ
Synonyms
Essential	no
Product	3-methylbutanoyl-CoA dehydrogenase
Function	mother cell metabolism, leucine utilization
Gene expression levels in SubtiExpress: yngJ
MW, pI	40 kDa, 5.794
Gene length, protein length	1140 bp, 380 aa
Immediate neighbours	yngI, ynzE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU18260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 3-methylbutanoyl-CoA --> 3-methylbut-2-enoyl-CoA PubMed

• Protein family:

• Paralogous protein(s): MmgC, AcdA, FadE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3OWA (from B. anthracis, 37% identity, 54% similarity)

• UniProt: O34421

• KEGG entry: [3]

• E.C. number: 1.3.99.10

Expression and regulation

• Operon: yngJ-yngI-yngH-yngHB-yngG-yngF-yngE PubMed

• Expression browser: yngJ PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)