Difference between revisions of "CshB"
(→Biological materials) |
|||
| Line 37: | Line 37: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<br/><br/> | <br/><br/> | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[DEAD-box RNA helicases]]}} | + | {{SubtiWiki category|[[DEAD-box RNA helicases]]}}, |
| + | {{SubtiWiki category|[[translation]]}} | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 56: | Line 53: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * poor growth at low temperatures (16 to 20°C) {{PubMed|23175651}} | ||
=== Database entries === | === Database entries === | ||
| Line 64: | Line 62: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| Line 114: | Line 110: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshB_2594184_2595500_-1 cshB] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshB_2594184_2595500_-1 cshB] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 150: | Line 146: | ||
=References= | =References= | ||
| − | |||
| − | |||
'''Additional publications:''' {{PubMed|20709848}} | '''Additional publications:''' {{PubMed|20709848}} | ||
| + | <pubmed>16352840,, 12399512, 20572937 23175651</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:00, 24 November 2012
- Description: DEAD-box RNA helicase
| Gene name | cshB |
| Synonyms | yqfR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Gene expression levels in SubtiExpress: cshB | |
| Interactions involving this protein in SubtInteract: CshB | |
| MW, pI | 49 kDa, 9.89 |
| Gene length, protein length | 1314 bp, 438 aa |
| Immediate neighbours | nfo, yqfQ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DEAD-box RNA helicases, translation
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25140
Phenotypes of a mutant
- poor growth at low temperatures (16 to 20°C) PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasma, colocalizes with the ribosomes PubMed
Database entries
- Structure:
- UniProt: P54475
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1051 (cat), available in Jörg Stülke's lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1064 (spc), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1011 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Additional publications: PubMed
