Difference between revisions of "CdaR"
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* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | ** IPTG inducible expression of Strep-''ybbR'' in ''E. coli'': pGP2565 (in [[pGP172]]), available in [[Stülke]] lab | + | ** IPTG inducible expression of Strep-''ybbR'' in ''E. coli'': pGP2565 (in [[pGP172]]), available in [[Jörg Stülke]]'s lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
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* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab. Respective plasmids: pGP1989 and pGP1992. | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab. Respective plasmids: pGP1989 and pGP1992. | ||
| − | * '''Strep-tag construct:''' GP1330 (aphA3, based on [[pGP1460]]), available in [[Jörg Stülke]]'s lab | + | * '''Strep-tag construct:''' |
| + | ** GP1330 (aphA3, based on [[pGP1460]]), available in [[Jörg Stülke]]'s lab | ||
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 10:16, 31 October 2012
- Description: unknown
| Gene name | ybbR |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | unknown |
| Gene expression levels in SubtiExpress: ybbR | |
| Metabolic function and regulation of this protein in SubtiPathways: Murein recycling | |
| MW, pI | 52 kDa, 4.719 |
| Gene length, protein length | 1449 bp, 483 aa |
| Immediate neighbours | ybbP, glmM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01760
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34659
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- GP999 (ybbR::cat), available in Jörg Stülke's lab
- GP985 (ybbP-ybbR::cat), available in Jörg Stülke's lab
- Expression vector:
- IPTG inducible expression of Strep-ybbR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.
- Strep-tag construct:
- GP1330 (aphA3, based on pGP1460), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed

