CopA

• Description: copper-transporting ATPase, resistance to copper
  
  

Gene name	copA
Synonyms	yvgX
Essential	no
Product	copper transporting ATPase
Function	copper export, detoxification
Gene expression levels in SubtiExpress: copA
Interactions involving this protein in SubtInteract: CopA
Metabolic function and regulation of this protein in SubtiPathways: metal ion homeostasis
MW, pI	85 kDa, 5.484
Gene length, protein length	2409 bp, 803 aa
Immediate neighbours	cadA, copZ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), resistance against toxic metals, membrane proteins

This gene is a member of the following regulons

CsoR regulon

The gene

Basic information

• Locus tag: BSU33500

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + Cu¹⁺(In) = ADP + phosphate + Cu¹⁺(Out) (according to Swiss-Prot)

• Protein family: Type IB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • two N-terminal soluble domains, CopAa and CopAb, connected by a short linker

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CopA-CopZ, for the transfer of the Cu(I) cluster PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 2RML ( N-terminal soluble domain)

• UniProt: O32220

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: copZ-copA PubMed

• Expression browser: copA PubMed

• Sigma factor: SigA PubMed
• Regulation:
  • induced by copper (CsoR) PubMed

• Regulatory mechanism:
  • CsoR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

References

Reviews

Amy C Rosenzweig, José M Argüello
Toward a molecular understanding of metal transport by P(1B)-type ATPases.
Curr Top Membr: 2012, 69;113-36
[PubMed:23046649] [WorldCat.org] [DOI] (I p)

Amie K Boal, Amy C Rosenzweig
Structural biology of copper trafficking.
Chem Rev: 2009, 109(10);4760-79
[PubMed:19824702] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed