Difference between revisions of "Eno"

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(Biological materials)
Line 152: Line 152:
  
 
* '''Mutant:'''  
 
* '''Mutant:'''  
** GP594 (''eno''::''cat''), available in [[Stülke]] lab  
+
** GP594 (''eno''::''cat''), available in [[Jörg Stülke]]'s lab  
** GP599 (''eno''::''erm''), available in [[Stülke]] lab  
+
** GP599 (''eno''::''erm''), available in [[Jörg Stülke]]'s lab  
** GP698 (''eno''-''[[pgm]]''::''cat''), available in [[Stülke]] lab
+
** GP698 (''eno''-''[[pgm]]''::''cat''), available in [[Jörg Stülke]]'s lab
** GP1215 (''eno''-''Strep'' ''(spc)''), purification from ''B. subtilis'', for [[SPINE]], available in [[Stülke]] lab  
 
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  
** pGP1426 (expression of ''[[eno]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
+
** pGP1426 (expression of ''[[eno]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
** pGP1500 (expression of ''[[pgm]]'' and ''[[eno]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
+
** pGP1500 (expression of ''[[pgm]]'' and ''[[eno]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
** pGP563 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab  
+
** pGP563 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab  
** pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Stülke]] lab
+
** pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab
** pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab  
+
** pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Jörg Stülke]]'s lab
 +
** GP1215 (chromosomal ''eno''-''Strep'' fusion, ''spc''), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab  
  
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
 
** see ''[[pgk]]''
 
** see ''[[pgk]]''
  
* '''GFP fusion:''' pHT315-yfp-eno, available in [[Mijakovic]] lab
+
* '''GFP fusion:'''  
 +
** pHT315-yfp-eno, available in [[Mijakovic]] lab
  
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
+
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
  
* '''FLAG-tag construct:''' GP1214 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
+
* '''FLAG-tag construct:'''  
 +
** GP1214 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
  
* '''Antibody:''' available in [[Stülke]] lab
+
* '''Antibody:''' available in [[Jörg Stülke]]'s lab
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 12:21, 1 October 2012

Gene name eno
Synonyms
Essential no
Product enolase
Function enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: eno
Interactions involving this protein in SubtInteract: Eno
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 46,4 kDa, 4.49
Gene length, protein length 1290 bp, 430 amino acids
Immediate neighbours yvbK, pgm
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Eno context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Eno expression.png





























Categories containing this gene/protein

carbon core metabolism, membrane proteins, phosphoproteins, universally conserved proteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

  • Locus tag: BSU33900

Phenotypes of a mutant

  • no growth on LB, requires glucose and malate
  • essential according to Kobayashi et al. on LB PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
  • Protein family: enolase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: reversible Michaelis-Menten PubMed
  • Domains:
    • substrate binding domain (366–369)
  • Modification: phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 PubMed, PubMed, PubMed
  • Cofactor(s): Mg2+
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: transcription repression by CggR PubMed
  • Additional information:

Biological materials

  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

Reviews

G H Reed, R R Poyner, T M Larsen, J E Wedekind, I Rayment
Structural and mechanistic studies of enolase.
Curr Opin Struct Biol: 1996, 6(6);736-43
[PubMed:8994873] [WorldCat.org] [DOI] (P p)

Subcellular localization of enolase

Additional publications: PubMed


Other original publications