Difference between revisions of "McpB"
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* '''Operon:''' ''[[mcpB]]'' {{PubMed|8188684}} | * '''Operon:''' ''[[mcpB]]'' {{PubMed|8188684}} | ||
− | * '''[ | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcpB_3210445_3212433_-1 mcpB] {{PubMed|22383849}} |
+ | |||
+ | * '''Sigma factor:''' [[SigD]] {{PubMed|8188684}} | ||
* '''Regulation:''' | * '''Regulation:''' |
Revision as of 16:11, 16 April 2012
- Description: membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, receptor for asparagine
Gene name | mcpB |
Synonyms | |
Essential | no |
Product | methyl-accepting chemotaxis protein |
Function | control of chemotaxis |
Interactions involving this protein in SubtInteract: McpB | |
MW, pI | 71 kDa, 5.13 |
Gene length, protein length | 1986 bp, 662 aa |
Immediate neighbours | tlpA, tgl |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
motility and chemotaxis, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31260
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): McpA
Extended information on the protein
- Kinetic information: K(D) for asparagine: 14 myM PubMed
- Domains:
- Modification: methylated at residues Gln371, Glu630, and Glu637 PubMed
- Cofactor(s):
- Effectors of protein activity: asparagine (binds to the part of McpB that is exposed to the exterior) PubMed
Database entries
- Structure:
- UniProt: P39215
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- in minimal medium, McpB is present with 6,200 +/- 1,900 molecules per cell PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776]
[WorldCat.org]
[DOI]
(I p)
Kang Wu, Hanna E Walukiewicz, George D Glekas, George W Ordal, Christopher V Rao
Attractant binding induces distinct structural changes to the polar and lateral signaling clusters in Bacillus subtilis chemotaxis.
J Biol Chem: 2011, 286(4);2587-95
[PubMed:21098025]
[WorldCat.org]
[DOI]
(I p)
George D Glekas, Joseph R Cates, Theodore M Cohen, Christopher V Rao, George W Ordal
Site-specific methylation in Bacillus subtilis chemotaxis: effect of covalent modifications to the chemotaxis receptor McpB.
Microbiology (Reading): 2011, 157(Pt 1);56-65
[PubMed:20864474]
[WorldCat.org]
[DOI]
(I p)
George D Glekas, Richard M Foster, Joseph R Cates, Jeffrey A Estrella, Michael J Wawrzyniak, Christopher V Rao, George W Ordal
A PAS domain binds asparagine in the chemotaxis receptor McpB in Bacillus subtilis.
J Biol Chem: 2010, 285(3);1870-8
[PubMed:19864420]
[WorldCat.org]
[DOI]
(I p)
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)
Hendrik Szurmant, Michael W Bunn, Stephen H Cho, George W Ordal
Ligand-induced conformational changes in the Bacillus subtilis chemoreceptor McpB determined by disulfide crosslinking in vivo.
J Mol Biol: 2004, 344(4);919-28
[PubMed:15544802]
[WorldCat.org]
[DOI]
(P p)
Michael W Bunn, George W Ordal
Receptor conformational changes enhance methylesterase activity during chemotaxis by Bacillus subtilis.
Mol Microbiol: 2004, 51(3);721-8
[PubMed:14731274]
[WorldCat.org]
[DOI]
(P p)
Michael W Bunn, George W Ordal
Transmembrane organization of the Bacillus subtilis chemoreceptor McpB deduced by cysteine disulfide crosslinking.
J Mol Biol: 2003, 331(4);941-9
[PubMed:12909020]
[WorldCat.org]
[DOI]
(P p)
Michael A Zimmer, Hendrik Szurmant, Michael M Saulmon, Marissa A Collins, Jason S Bant, George W Ordal
The role of heterologous receptors in McpB-mediated signalling in Bacillus subtilis chemotaxis.
Mol Microbiol: 2002, 45(2);555-68
[PubMed:12123464]
[WorldCat.org]
[DOI]
(P p)
J R Kirby, C J Kristich, M M Saulmon, M A Zimmer, L F Garrity, I B Zhulin, G W Ordal
CheC is related to the family of flagellar switch proteins and acts independently from CheD to control chemotaxis in Bacillus subtilis.
Mol Microbiol: 2001, 42(3);573-85
[PubMed:11722727]
[WorldCat.org]
[DOI]
(P p)
M A Zimmer, J Tiu, M A Collins, G W Ordal
Selective methylation changes on the Bacillus subtilis chemotaxis receptor McpB promote adaptation.
J Biol Chem: 2000, 275(32);24264-72
[PubMed:10825179]
[WorldCat.org]
[DOI]
(P p)
J R Kirby, M M Saulmon, C J Kristich, G W Ordal
CheY-dependent methylation of the asparagine receptor, McpB, during chemotaxis in Bacillus subtilis.
J Biol Chem: 1999, 274(16);11092-100
[PubMed:10196193]
[WorldCat.org]
[DOI]
(P p)
J R Kirby, C J Kristich, S L Feinberg, G W Ordal
Methanol production during chemotaxis to amino acids in Bacillus subtilis.
Mol Microbiol: 1997, 24(4);869-78
[PubMed:9194713]
[WorldCat.org]
[DOI]
(P p)
D W Hanlon, G W Ordal
Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis.
J Biol Chem: 1994, 269(19);14038-46
[PubMed:8188684]
[WorldCat.org]
(P p)
D W Hanlon, C Ying, G W Ordal
Purification and reconstitution of the methyl-accepting chemotaxis proteins from Bacillus subtilis.
Biochim Biophys Acta: 1993, 1158(3);345-51
[PubMed:8251536]
[WorldCat.org]
[DOI]
(P p)
M S Thoelke, J M Casper, G W Ordal
Methyl group turnover on methyl-accepting chemotaxis proteins during chemotaxis by Bacillus subtilis.
J Biol Chem: 1990, 265(4);1928-32
[PubMed:2105313]
[WorldCat.org]
(P p)
M S Thoelke, J R Kirby, G W Ordal
Novel methyl transfer during chemotaxis in Bacillus subtilis.
Biochemistry: 1989, 28(13);5585-9
[PubMed:2505839]
[WorldCat.org]
[DOI]
(P p)
J A Ahlgren, G W Ordal
Methyl esterification of glutamic acid residues of methyl-accepting chemotaxis proteins in Bacillus subtilis.
Biochem J: 1983, 213(3);759-63
[PubMed:6137212]
[WorldCat.org]
[DOI]
(P p)