GuaD

• Description: guanine deaminase, general stress protein, important for survival of ethanol stress
  
  

Gene name	guaD
Synonyms	gde, yknA
Essential	no
Product	guanine deaminase
Function	deamination of guanine to xanthine, purine salvage and interconversion
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Purine catabolism, Nucleotides (regulation)
MW, pI	17 kDa, 4.999
Gene length, protein length	468 bp, 156 aa
Immediate neighbours	ohrB, metE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

PucR regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU13170

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Guanine + H₂O = xanthine + NH₃ (according to Swiss-Prot)

• Protein family: cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1WKQ

• UniProt: O34598

• KEGG entry: [3]

• E.C. number: 3.5.4.3

Expression and regulation

• Operon: guaD PubMed

• Sigma factor: SigA PubMed, SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: