Difference between revisions of "LytF"
(→Extended information on the protein) |
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* '''Protein family:''' nlpC/p60 family (according to Swiss-Prot) | * '''Protein family:''' nlpC/p60 family (according to Swiss-Prot) | ||
| − | * '''Paralogous protein(s):''' | + | * '''Paralogous protein(s):''' the C-terminal D,L-endopeptidase domains of [[LytE]], [[LytF]], [[CwlS]], and [[CwlO]] exhibit strong sequence similarity |
=== Extended information on the protein === | === Extended information on the protein === | ||
| Line 76: | Line 76: | ||
* '''Domains:''' | * '''Domains:''' | ||
| + | ** C-terminal D,L-endopeptidase domain {{PubMed|22139507}} | ||
* '''Modification:''' | * '''Modification:''' | ||
| Line 86: | Line 87: | ||
** [[IseA]]-[[LytF]] (catalytic domain) {{PubMed|18761696}} | ** [[IseA]]-[[LytF]] (catalytic domain) {{PubMed|18761696}} | ||
| − | * '''[[Localization]]:''' secreted (according to Swiss-Prot) | + | * '''[[Localization]]:''' |
| + | **secreted (according to Swiss-Prot) | ||
| + | ** localizes to cell septa and poles {{PubMed|22139507}} | ||
=== Database entries === | === Database entries === | ||
| Line 133: | Line 136: | ||
=References= | =References= | ||
| − | <pubmed> 20351052,10322020,10206711,14594841,18761696, 19542270 </pubmed> | + | <pubmed> 20351052,10322020,10206711,14594841,18761696, 19542270 22139507</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:59, 8 December 2011
- Description: gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
| Gene name | lytF |
| Synonyms | cwlE, yhdD |
| Essential | no |
| Product | gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin) |
| Function | cell separation |
| Interactions involving this protein in SubtInteract: LytF | |
| MW, pI | 51 kDa, 10.41 |
| Gene length, protein length | 1464 bp, 488 aa |
| Immediate neighbours | yhdC, nsrR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
cell wall degradation/ turnover
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU09370
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: nlpC/p60 family (according to Swiss-Prot)
- Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity
Extended information on the protein
- Kinetic information:
- Domains:
- C-terminal D,L-endopeptidase domain PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- secreted (according to Swiss-Prot)
- localizes to cell septa and poles PubMed
Database entries
- Structure:
- UniProt: O07532
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Masayuki Hashimoto, Seika Ooiwa, Junichi Sekiguchi
Synthetic lethality of the lytE cwlO genotype in Bacillus subtilis is caused by lack of D,L-endopeptidase activity at the lateral cell wall.
J Bacteriol: 2012, 194(4);796-803
[PubMed:22139507]
[WorldCat.org]
[DOI]
(I p)
Yunrong Chai, Thomas Norman, Roberto Kolter, Richard Losick
An epigenetic switch governing daughter cell separation in Bacillus subtilis.
Genes Dev: 2010, 24(8);754-65
[PubMed:20351052]
[WorldCat.org]
[DOI]
(I p)
Rui Chen, Sarah B Guttenplan, Kris M Blair, Daniel B Kearns
Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.
J Bacteriol: 2009, 191(18);5775-84
[PubMed:19542270]
[WorldCat.org]
[DOI]
(I p)
Hiroki Yamamoto, Yukiko Miyake, Miharu Hisaoka, Shin-Ichirou Kurosawa, Junichi Sekiguchi
The major and minor wall teichoic acids prevent the sidewall localization of vegetative DL-endopeptidase LytF in Bacillus subtilis.
Mol Microbiol: 2008, 70(2);297-310
[PubMed:18761696]
[WorldCat.org]
[DOI]
(I p)
Hiroki Yamamoto, Shin-ichirou Kurosawa, Junichi Sekiguchi
Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.
J Bacteriol: 2003, 185(22);6666-77
[PubMed:14594841]
[WorldCat.org]
[DOI]
(P p)
R Ohnishi, S Ishikawa, J Sekiguchi
Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.
J Bacteriol: 1999, 181(10);3178-84
[PubMed:10322020]
[WorldCat.org]
[DOI]
(P p)
Philippe Margot, Marco Pagni, Dimitri Karamata
Bacillus subtilis 168 gene lytF encodes a gamma-D-glutamate-meso-diaminopimelate muropeptidase expressed by the alternative vegetative sigma factor, sigmaD.
Microbiology (Reading): 1999, 145 ( Pt 1);57-65
[PubMed:10206711]
[WorldCat.org]
[DOI]
(P p)
