Difference between revisions of "LytE"
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| − | * '''Description:''' cell wall hydrolase (major autolysin),endopeptidase-type autolysin <br/><br/> | + | * '''Description:''' cell wall hydrolase (major autolysin), D,L-endopeptidase-type autolysin <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Product''' || cell wall hydrolase (major autolysin),endopeptidase-type autolysin | |style="background:#ABCDEF;" align="center"| '''Product''' || cell wall hydrolase (major autolysin),endopeptidase-type autolysin | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || major autolysin, cell separation, cell | + | |style="background:#ABCDEF;" align="center"|'''Function''' || major autolysin, cell separation, cell proliferation |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 10.713 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 10.713 | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | ** a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128}} | + | ** a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128,22139507}} |
** growth defect at high temperature {{PubMed|21541672}} | ** growth defect at high temperature {{PubMed|21541672}} | ||
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* '''Domains:''' | * '''Domains:''' | ||
| + | ** C-terminal D,L-endopeptidase domain {{PubMed|22139507}} | ||
* '''Modification:''' | * '''Modification:''' | ||
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** [[MreBH]]-[[LytE]] {{PubMed|16950129}} | ** [[MreBH]]-[[LytE]] {{PubMed|16950129}} | ||
| − | * '''[[Localization]]:''' secreted (according to Swiss-Prot) | + | * '''[[Localization]]:''' |
| + | ** secreted (according to Swiss-Prot) | ||
| + | ** binds the cell wall {{PubMed|21261835}} | ||
| + | ** localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) {{PubMed|22139507}} | ||
=== Database entries === | === Database entries === | ||
Revision as of 11:58, 8 December 2011
- Description: cell wall hydrolase (major autolysin), D,L-endopeptidase-type autolysin
| Gene name | lytE |
| Synonyms | papQ, cwlF |
| Essential | no |
| Product | cell wall hydrolase (major autolysin),endopeptidase-type autolysin |
| Function | major autolysin, cell separation, cell proliferation |
| MW, pI | 37 kDa, 10.713 |
| Gene length, protein length | 1029 bp, 343 aa |
| Immediate neighbours | phoA, citR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
cell wall degradation/ turnover
This gene is a member of the following regulons
SigH regulon, SigI regulon, Spo0A regulon, WalR regulon
The gene
Basic information
- Locus tag: BSU09420
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: nlpC/p60 family (according to Swiss-Prot)
- Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity
Extended information on the protein
- Kinetic information:
- Domains:
- C-terminal D,L-endopeptidase domain PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P54421
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: lytE PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
