Difference between revisions of "GltB"
| Line 92: | Line 92: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2VDC 2VDC] (the [[GltA]]-[[GltB]] complex of ''Azospirillum brasiliense'') {{PubMed|18199747}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34399 O34399] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O34399 O34399] | ||
| Line 139: | Line 139: | ||
=References= | =References= | ||
| − | <pubmed>12823818,20933603,11029411,7559360 15150225 2548995 17183217 17608797 17134717 14523131, 12823818 , 18326565 </pubmed> | + | <pubmed>12823818,20933603,11029411,7559360 15150225 2548995 17183217 17608797 17134717 14523131, 12823818 , 18326565 18199747</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:17, 9 December 2010
- Description: small subunit of glutamate synthase
| Gene name | gltB |
| Synonyms | |
| Essential | no |
| Product | glutamate synthase (small subunit) |
| Function | glutamate biosynthesis |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
| MW, pI | 54.6 kDa, 7.69 |
| Gene length, protein length | 1479 bp, 493 amino acids |
| Immediate neighbours | yogA, gltA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU18440
Phenotypes of a mutant
auxotrophic for glutamate
Database entries
- DBTBS entry: [1]
- SubtiList entry:[2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH
- Protein family: glutamate synthase family.
- Paralogous protein(s): none
Extended information on the protein
- Kinetic information:
- Domains:
- nucleotide binding domain (NADP) (299–313)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: O34399
- KEGG entry: [3]
- E.C. number: 1.4.1.13
Additional information
Expression and regulation
- Regulation: see gltA
- Additional information:
Biological materials
- Mutant: GP807 (del gltAB::tet), GP517 (ermC), both available in Stülke lab
- Expression vector:
- lacZ fusion: see gltA
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
