MtnD

• Description: 1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase
  
  

Gene name	mtnD
Synonyms	ykrZ
Essential	no
Product	1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Function	methionine salvage
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	20 kDa, 4.408
Gene length, protein length	534 bp, 178 aa
Immediate neighbours	mtnB, ykvA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU13620

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O₂ = 3-(methylthio)propanoate + formate + CO (according to Swiss-Prot)

• Protein family: acireductone dioxygenase (ARD) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31669

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtnW-mtnX-mtnB-mtnD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: