RecA

• Description: multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
  
  

Gene name	recA
Synonyms	recE
Essential	no
Product	multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
Function	homologous recombination, DNA repair
MW, pI	37 kDa, 4.883
Gene length, protein length	1041 bp, 347 aa
Immediate neighbours	cinA, pbpX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU16940

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

RecA filaments are dismantled from DNA by PcrA PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: recA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Ser-2 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RecA-DprA PubMed, RecA-RecU PubMed

• Localization: cytoplasm (according to Swiss-Prot), Nucleoid (Mid-cell) PubMed

Database entries

• Structure:

• UniProt: P16971

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by DNA damage (LexA) PubMed
  • induced by conditions that trigger development of genetic competence (ComK) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: IRN444 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Reviews

Justin Courcelle, Philip C Hanawalt
RecA-dependent recovery of arrested DNA replication forks.
Annu Rev Genet: 2003, 37;611-46
[PubMed:14616075] [WorldCat.org] [DOI] (P p)

Michael M Cox
The bacterial RecA protein as a motor protein.
Annu Rev Microbiol: 2003, 57;551-77
[PubMed:14527291] [WorldCat.org] [DOI] (P p)

Shelley L Lusetti, Michael M Cox
The bacterial RecA protein and the recombinational DNA repair of stalled replication forks.
Annu Rev Biochem: 2002, 71;71-100
[PubMed:12045091] [WorldCat.org] [DOI] (P p)

M M Cox
Recombinational DNA repair in bacteria and the RecA protein.
Prog Nucleic Acid Res Mol Biol: 1999, 63;311-66
[PubMed:10506835] [WorldCat.org] [DOI] (P p)