FolE2

• Description: GTP cyclohydrolase IB, replaces FolE under conditions of zinc starvation
  
  

Gene name	folE2
Synonyms	yciA
Essential	no
Product	zinc-independent GTP cyclohydrolase IB
Function	biosynthesis of folate
MW, pI	34 kDa, 7.831
Gene length, protein length	915 bp, 305 aa
Immediate neighbours	nasA, yciB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03340

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis of cofactors

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: GTP + H₂O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate (according to Swiss-Prot)

• Protein family: GTP cyclohydrolase IV family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): activated by a variety of divalent cations PubMed

• Effectors of protein activity:

• Interactions: homotetrameric PubMed

• Localization:

Database entries

• Structure: 3D1T PubMed

• UniProt: P94398

• KEGG entry: [3]

• E.C. number: 3.5.4.16

Expression and regulation

• Operon: folE2-yciB-yciC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by zinc starvation (Zur) PubMed

• Regulatory mechanism:
  • Zur: transcription repression in the presence of zinc PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: