Difference between revisions of "CapE"
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| − | * '''Description:''' | + | * '''Description:''' stimulates poly-gamma-glutamate production in the presence of zinc<br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Product''' || unknown | |style="background:#ABCDEF;" align="center"| '''Product''' || unknown | ||
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || capsule synthesis |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 6.487 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 6.487 | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' stimulates poly-gamma-glutamate production in the presence of zinc {{PubMed|20812257}} |
* '''Protein family:''' | * '''Protein family:''' | ||
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=References= | =References= | ||
| − | <pubmed>,19420703, 16233197 16267300 20564357 20564574 </pubmed> | + | <pubmed>,19420703, 16233197 16267300 20564357 20564574 20812257 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:45, 3 September 2010
- Description: stimulates poly-gamma-glutamate production in the presence of zinc
| Gene name | capE |
| Synonyms | ywtC, pgsE |
| Essential | no |
| Product | unknown |
| Function | capsule synthesis |
| MW, pI | 6 kDa, 6.487 |
| Gene length, protein length | 165 bp, 55 aa |
| Immediate neighbours | pgdS, capA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU35870
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: stimulates poly-gamma-glutamate production in the presence of zinc PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P96739
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Daisuke Yamashiro, Megumi Yoshioka, Makoto Ashiuchi
Bacillus subtilis pgsE (Formerly ywtC) stimulates poly-γ-glutamate production in the presence of zinc.
Biotechnol Bioeng: 2011, 108(1);226-30
[PubMed:20812257]
[WorldCat.org]
[DOI]
(I p)
Tohru Kamei, Daisuke Yamashiro, Terumi Horiuchii, Yutaka Minouchi, Makoto Ashiuchi
Identification and biochemical characterization of membranous short-chain polyglutamate from Bacillus subtilis.
Chem Biodivers: 2010, 7(6);1563-72
[PubMed:20564574]
[WorldCat.org]
[DOI]
(I p)
Chuan-Mei Yeh, Jyh-Perng Wang, Shih-Ching Lo, Wen-Chia Chan, Ming-Yi Lin
Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a Bacillus subtilis strain.
Biotechnol Prog: 2010, 26(4);1001-7
[PubMed:20564357]
[WorldCat.org]
[DOI]
(I p)
Keitarou Kimura, Lam-Son Phan Tran, Thi-Huyen Do, Yoshifumi Itoh
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci Biotechnol Biochem: 2009, 73(5);1149-55
[PubMed:19420703]
[WorldCat.org]
[DOI]
(I p)
Thomas Candela, Michèle Mock, Agnès Fouet
CapE, a 47-amino-acid peptide, is necessary for Bacillus anthracis polyglutamate capsule synthesis.
J Bacteriol: 2005, 187(22);7765-72
[PubMed:16267300]
[WorldCat.org]
[DOI]
(P p)
Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Difference in transcription levels of cap genes for gamma-polyglutamic acid production between Bacillus subtilis IFO 16449 and Marburg 168.
J Biosci Bioeng: 2002, 93(2);252-4
[PubMed:16233197]
[WorldCat.org]
[DOI]
(P p)
